RPS6KA5 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RPS6KA5.
Immunogen
A synthetic peptide corresponding to residues surrounding S360 of human RPS6KA5.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to RPS6KA5.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, treated with Sorbitol (400 mM, 30 mins), using RPS6KA5 polyclonal antibody (Cat # PAB18247).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using RPS6KA5 polyclonal antibody (Cat # PAB18247).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPS6KA5
Entrez GeneID
9252Protein Accession#
O75582Gene Name
RPS6KA5
Gene Alias
MGC1911, MSK1, MSPK1, RLPK
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 5
Omim ID
603607Gene Ontology
HyperlinkGene Summary
90kDa
Other Designations
mitogen- and stress-activated protein kinase 1|ribosomal protein S6 kinase, 90kD, polypeptide 5
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Light stimulates MSK1 activation in the suprachiasmatic nucleus via a PACAP-ERK/MAP kinase-dependent mechanism.
Butcher GQ, Lee B, Cheng HY, Obrietan K.
Journal of Neuroscience 2005 Jun; 25(22):5305.
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Tumor necrosis factor activates CRE-binding protein through a p38 MAPK/MSK1 signaling pathway in endothelial cells.
Gustin JA, Pincheira R, Mayo LD, Ozes ON, Kessler KM, Baerwald MR, Korgaonkar CK, Donner DB.
American Journal of Physiology - Cell Physiology 2004 Mar; 286(3):C547.
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MSK1 and MSK2 are required for the mitogen- and stress-induced phosphorylation of CREB and ATF1 in fibroblasts.
Wiggin GR, Soloaga A, Foster JM, Murray-Tait V, Cohen P, Arthur JS.
Molecular and Cellular Biology 2002 Apr; 22(8):2871.
Application:WB, Mouse, Mouse fibroblasts.
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Light stimulates MSK1 activation in the suprachiasmatic nucleus via a PACAP-ERK/MAP kinase-dependent mechanism.
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