MAP2K1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP2K1.
Immunogen
A synthetic peptide corresponding to residues surrounding T286 of human MAP2K1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to MAP2K1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250 ng/mL, 5 mins), using MAP2K1 polyclonal antibody (Cat # PAB18246).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAP2K1 polyclonal antibody (Cat # PAB18246).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAP2K1
Entrez GeneID
5604Protein Accession#
Q02750Gene Name
MAP2K1
Gene Alias
MAPKK1, MEK1, MKK1, PRKMK1
Gene Description
mitogen-activated protein kinase kinase 1
Omim ID
176872Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
Other Designations
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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Interactome
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Pathway
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Disease
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Publication Reference
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Constitutive activation of MEK1 in chondrocytes causes Stat1-independent achondroplasia-like dwarfism and rescues the Fgfr3-deficient mouse phenotype.
Murakami S, Balmes G, McKinney S, Zhang Z, Givol D, de Crombrugghe B.
Genes & Development 2004 Feb; 18(3):290.
Application:IS, NB, WB, WB-Tr, Mouse, Chondrocytes, C3H10T1/2 cells.
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Ubiquitin (UbC) expression in muscle cells is increased by glucocorticoids through a mechanism involving Sp1 and MEK1.
Marinovic AC, Zheng B, Mitch WE, Price SR.
The Journal of Biological Chemistry 2002 May; 277(19):16673.
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Wnt1 and MEK1 cooperate to promote cyclin D1 accumulation and cellular transformation.
Rimerman RA, Gellert-Randleman A, Diehl JA.
The Journal of Biological Chemistry 2000 May; 275(19):14736.
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Constitutive activation of MEK1 in chondrocytes causes Stat1-independent achondroplasia-like dwarfism and rescues the Fgfr3-deficient mouse phenotype.
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