EIF4G1 polyclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EIF4G1 polyclonal antibody (Cat # PAB18240).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using EIF4G1 polyclonal antibody (Cat # PAB18240).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF4G1.
Immunogen
A synthetic peptide corresponding to human EIF4G1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to EIF4G1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EIF4G1 polyclonal antibody (Cat # PAB18240).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using EIF4G1 polyclonal antibody (Cat # PAB18240).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF4G1
Entrez GeneID
1981Protein Accession#
Q04637Gene Name
EIF4G1
Gene Alias
DKFZp686A1451, EIF4F, EIF4G, p220
Gene Description
eukaryotic translation initiation factor 4 gamma, 1
Omim ID
600495Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a component of the protein complex EIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome. Alternative splicing results in five transcript variants encoding four distinct isoforms. [provided by RefSeq
Other Designations
EIF4-gamma
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Interactome
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Publication Reference
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Protection of cap-dependent protein synthesis in vivo and in vitro with an eIF4G-1 variant highly resistant to cleavage by Coxsackievirus 2A protease.
Zhao X, Lamphear BJ, Xiong D, Knowlton K, Rhoads RE.
The Journal of Biological Chemistry 2003 Feb; 278(7):4449.
Application：WB-Tr, Human, HeLa cells.
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The histone 3'-terminal stem-loop-binding protein enhances translation through a functional and physical interaction with eukaryotic initiation factor 4G (eIF4G) and eIF3.
Ling J, Morley SJ, Pain VM, Marzluff WF, Gallie DR.
Molecular and Cellular Biology 2002 Nov; 22(22):7853.
Application：IP-WB, Human, 293 cells.
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eIF4G functionally differs from eIFiso4G in promoting internal initiation, cap-independent translation, and translation of structured mRNAs.
Gallie DR, Browning KS.
The Journal of Biological Chemistry 2001 Oct; 276(40):36951.
Application：WB, Plant, Wheat germ lysates.
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Protection of cap-dependent protein synthesis in vivo and in vitro with an eIF4G-1 variant highly resistant to cleavage by Coxsackievirus 2A protease.
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