WAS polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using WAS polyclonal antibody (Cat # PAB18233).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using WAS polyclonal antibody (Cat # PAB18233).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of WAS.
Immunogen
A synthetic peptide corresponding to residues surrounding Y290 of human WAS.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to WAS.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using WAS polyclonal antibody (Cat # PAB18233).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using WAS polyclonal antibody (Cat # PAB18233).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — WAS
Entrez GeneID
7454Protein Accession#
P42768Gene Name
WAS
Gene Alias
IMD2, THC, WASP
Gene Description
Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
Gene Ontology
HyperlinkGene Summary
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq
Other Designations
OTTHUMP00000032395|Wiskott-Aldrich syndrome protein|thrombocytopenia 1 (X-linked)
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Interactome
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Pathway
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Disease
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Publication Reference
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Normal Arp2/3 complex activation in platelets lacking WASp.
Falet H, Hoffmeister KM, Neujahr R, Hartwig JH.
Blood 2002 Sep; 100(6):2113.
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Normal Arp2/3 complex activation in platelets lacking WASp.
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