SP1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SP1.
Immunogen
A synthetic peptide corresponding to residues surrounding T453 of human SP1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to SP1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from LoVo cells, using SP1 polyclonal antibody (Cat # PAB18222).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using SP1 polyclonal antibody (Cat # PAB18222).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using SP1 polyclonal antibody (Cat # PAB18222).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — SP1
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Interactome
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Pathway
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Disease
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Publication Reference
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Down-regulation of overexpressed sp1 protein in human fibrosarcoma cell lines inhibits tumor formation.
Lou Z, O'Reilly S, Liang H, Maher VM, Sleight SD, McCormick JJ.
Cancer Research 2005 Feb; 65(3):1007.
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Reversion of transcriptional repression of Sp1 by 5 aza-2' deoxycytidine restores TGF-beta type II receptor expression in the pancreatic cancer cell line MIA PaCa-2.
Venkatasubbarao K, Ammanamanchi S, Brattain MG, Mimari D, Freeman JW.
Cancer Research 2001 Aug; 61(16):6239.
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Sp1 plays a critical role in the transcriptional activation of the human cyclin-dependent kinase inhibitor p21(WAF1/Cip1) gene by the p53 tumor suppressor protein.
Koutsodontis G, Tentes I, Papakosta P, Moustakas A, Kardassis D.
The Journal of Biological Chemistry 2001 Aug; 276(31):29116.
Application:IP, WB-Tr, Monkey, COS-7 cells.
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Down-regulation of overexpressed sp1 protein in human fibrosarcoma cell lines inhibits tumor formation.
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