SOX9 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SOX9.
Immunogen
A synthetic peptide corresponding to residues surrounding S181 of human SOX9.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to SOX9.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with PBS (60 mins), using SOX9 polyclonal antibody (Cat # PAB18221).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using SOX9 polyclonal antibody (Cat # PAB18221).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using SOX9 polyclonal antibody (Cat # PAB18221).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — SOX9
Entrez GeneID
6662Protein Accession#
P48436Gene Name
SOX9
Gene Alias
CMD1, CMPD1, SRA1
Gene Description
SRY (sex determining region Y)-box 9
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq
Other Designations
SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal)|SRY (sex-determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal)|SRY (sex-determining region Y)-box 9 protein|campomelic dysplasia, autosomal sex-revers
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Interactome
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Disease
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Publication Reference
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c-Jun/activator protein-1 mediates interleukin-1beta-induced dedifferentiation but not cyclooxygenase-2 expression in articular chondrocytes.
Hwang SG, Yu SS, Poo H, Chun JS.
The Journal of Biological Chemistry 2005 Aug; 280(33):29780.
Application:WB, Rabbit, Rabbit articular chondrocytes.
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Follicular cells acquire sertoli cell characteristics after oocyte loss.
Guigon CJ, Coudouel N, Mazaud-Guittot S, Forest MG, Magre S.
Endocrinology 2005 Jul; 146(7):2992.
Application:IHC-P, Rat, Rat oocyte-depleted follicle.
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Chemotactic role of neurotropin 3 in the embryonic testis that facilitates male sex determination.
Cupp AS, Uzumcu M, Skinner MK.
Biology of Reproduction 2003 Jun; 68(6):2033.
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c-Jun/activator protein-1 mediates interleukin-1beta-induced dedifferentiation but not cyclooxygenase-2 expression in articular chondrocytes.
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