RHOA polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RHOA.
Immunogen
A synthetic peptide corresponding to residues surrounding S118 of human RHOA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to RHOA.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using RHOA polyclonal antibody (Cat # PAB18218).
Peptide "+" means "peptide blocking".Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RHOA
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Interactome
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Pathway
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Disease
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Publication Reference
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Obesogenic diet-induced gut barrier dysfunction and pathobiont expansion aggravate experimental colitis.
Lee JC, Lee HY, Kim TK, Kim MS, Park YM, Kim J, Park K, Kweon MN, Kim SH, Bae JW, Hur KY, Lee MS.
PLoS One 2017 Nov; 12(11):e0187515.
Application:IHC-P, Mouse, Mouse small intestine.
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, K
Genome Research 2004 Oct; 14(10B):2121.
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Obesogenic diet-induced gut barrier dysfunction and pathobiont expansion aggravate experimental colitis.
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