PRKACA polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKACA.
Immunogen
A synthetic peptide corresponding to residues surrounding T197 of human PRKACA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PRKACA.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of extracts from mouse brain cells, using PRKACA polyclonal antibody (Cat # PAB18208).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PRKACA polyclonal antibody (Cat # PAB18208).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using PRKACA polyclonal antibody (Cat # PAB18208).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKACA
Entrez GeneID
5566Protein Accession#
P17612Gene Name
PRKACA
Gene Alias
MGC102831, MGC48865, PKACA
Gene Description
protein kinase, cAMP-dependent, catalytic, alpha
Omim ID
601639Gene Ontology
HyperlinkGene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
PKA C-alpha|cAMP-dependent protein kinase catalytic subunit alpha|cAMP-dependent protein kinase catalytic subunit alpha, isoform 1|protein kinase A catalytic subunit
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Gene Info — PRKACB
Entrez GeneID
5567Protein Accession#
P17612Gene Name
PRKACB
Gene Alias
DKFZp781I2452, MGC41879, MGC9320, PKACB
Gene Description
protein kinase, cAMP-dependent, catalytic, beta
Omim ID
176892Gene Ontology
HyperlinkGene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000011663|OTTHUMP00000011664|OTTHUMP00000011666|PKA C-beta|cAMP-dependent protein kinase catalytic beta subunit isoform 4ab|cAMP-dependent protein kinase catalytic subunit beta|protein kinase A catalytic subunit beta
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Interactome
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Publication Reference
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Identification of ChChd3 as a novel substrate of the cAMP-dependent protein kinase (PKA) using an analog-sensitive catalytic subunit.
Schauble S, King CC, Darshi M, Koller A, Shah K, Taylor SS.
The Journal of Biological Chemistry 2007 May; 282(20):14952.
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Identification of ChChd3 as a novel substrate of the cAMP-dependent protein kinase (PKA) using an analog-sensitive catalytic subunit.
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