PEA15 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PEA15.
Immunogen
A synthetic peptide corresponding to residues surrounding S116 of human PEA15.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PEA15.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells, treated with PMA (125 ng/mL, 30 mins), using PEA15 polyclonal antibody (Cat # PAB18206).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using PEA15 polyclonal antibody (Cat # PAB18206).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using PEA15 polyclonal antibody (Cat # PAB18206).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PEA15
Entrez GeneID
8682Protein Accession#
Q15121Gene Name
PEA15
Gene Alias
HMAT1, HUMMAT1H, MAT1, MAT1H, PEA-15, PED
Gene Description
phosphoprotein enriched in astrocytes 15
Omim ID
603434Gene Ontology
HyperlinkGene Summary
PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.[supplied by OMIM
Other Designations
OTTHUMP00000029886|Phosphoprotein enriched in astrocytes, 15kD|homolog of mouse MAT-1 oncogene
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Interactome
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Disease
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Publication Reference
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PEA-15 inhibits tumor cell invasion by binding to extracellular signal-regulated kinase 1/2.
Glading A, Koziol JA, Krueger J, Ginsberg MH.
Cancer Research 2007 Feb; 67(4):1536.
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Phosphorylation of phosphoprotein enriched in astrocytes (PEA-15) regulates extracellular signal-regulated kinase-dependent transcription and cell proliferation.
Krueger J, Chou FL, Glading A, Schaefer E, Ginsberg MH.
Molecular Biology of the Cell 2005 Aug; 16(8):3552.
Application:IHC-P, WB-Tr, Human, Mouse, CHO, Human mammary tissues, MDA-MB-231, NIH/3T3 cells.
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Protein kinase B/Akt binds and phosphorylates PED/PEA-15, stabilizing its antiapoptotic action.
Trencia A, Perfetti A, Cassese A, Vigliotta G, Miele C, Oriente F, Santopietro S, Giacco F, Condorelli G, Formisano P, Beguinot F.
Molecular and Cellular Biology 2003 Jul; 23(13):4511.
Application:WB-Ce, WB-Tr, Human, 293 cells.
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PEA-15 inhibits tumor cell invasion by binding to extracellular signal-regulated kinase 1/2.
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