PAK1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PAK1.
Immunogen
A synthetic peptide corresponding to residues surrounding T212 of human PAK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PAK1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, treated with Etoposide (25 uM, 60 mins), using PAK1 polyclonal antibody (Cat # PAB18201).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PAK1 polyclonal antibody (Cat # PAB18201).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using PAK1 polyclonal antibody (Cat # PAB18201).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
-
Gene Info — PAK1
Entrez GeneID
5058Protein Accession#
Q13153Gene Name
PAK1
Gene Alias
MGC130000, MGC130001, PAKalpha
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 1
Omim ID
602590Gene Ontology
HyperlinkGene Summary
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
STE20 homolog, yeast|p21-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Phosphorylation of Pak1 by the p35/Cdk5 kinase affects neuronal morphology.
Rashid T, Banerjee M, Nikolic M.
The Journal of Biological Chemistry 2001 Dec; 276(52):49043.
Application:ICC, IF, IP, WB-Tr, Monkey, Rat, COS-1, COS-7 cells, Rat embryo cortical neurons, Rat primary hippocampal neurons.
-
p21-activated kinase (PAK1) is phosphorylated and activated by 3-phosphoinositide-dependent kinase-1 (PDK1).
King CC, Gardiner EM, Zenke FT, Bohl BP, Newton AC, Hemmings BA, Bokoch GM.
The Journal of Biological Chemistry 2000 Dec; 275(52):41201.
-
Phosphorylation of Pak1 by the p35/Cdk5 kinase affects neuronal morphology.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com