NOS1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from Raw 264.7 cells, treated with INF (2500 U/mL, 10 mins), using NOS1 polyclonal antibody (Cat # PAB18194).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using NOS1 polyclonal antibody (Cat # PAB18194).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NOS1.
Immunogen
A synthetic peptide corresponding to residues surrounding S852 of human NOS1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to NOS1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Raw 264.7 cells, treated with INF (2500 U/mL, 10 mins), using NOS1 polyclonal antibody (Cat # PAB18194).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using NOS1 polyclonal antibody (Cat # PAB18194).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — NOS1
Entrez GeneID
4842Protein Accession#
P29475Gene Name
NOS1
Gene Alias
IHPS1, NOS, nNOS
Gene Description
nitric oxide synthase 1 (neuronal)
Gene Ontology
HyperlinkGene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. This gene encodes a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in this gene are associated with infantile hypertrophic pyloric stenosis type 1
Other Designations
neuronal nitric oxide synthase
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