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Last updated: 2016/12/4
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MITF polyclonal antibody

  • Catalog # : PAB18189
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MITF.
  • Immunogen:
  • A synthetic peptide corresponding to residues surrounding S180/S73 of human MITF.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is specific to MITF.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Concentration:
  • 1 mg/mL
  • Recommend Usage:
  • Western Blot (1:500-1:1000)
    Immunohistochemistry (1:50-1:100)
    Immunofluorescence (1:500-1:1000)
    ELISA (1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • 2.
  • Genetics. 2004 May;167(1):233-41.
    Hallsson JH, Haflidadottir BS, Stivers C, Odenwald W, Arnheiter H, Pignoni F, Steingrimsson E.Genetics. 2004 May;167(1):233-41.
  • 3.
  • Blood. 2001 Apr 1;97(7):2038-44.
    Morii E, Ogihara H, Kim DK, Ito A, Oboki K, Lee YM, Jippo T, Nomura S, Maeyama K, Lamoreux ML, Kitamura Y.Blood. 2001 Apr 1;97(7):2038-44.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from HepG2 cells (Lane 1) and COLO 205 cells (Lane 2 and 3), using MITF polyclonal antibody (Cat # PAB18189).
    Peptide "+" means "peptide blocking".
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using MITF polyclonal antibody (Cat # PAB18189).
    Peptide "+" means "peptide blocking".
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells, using MITF polyclonal antibody (Cat # PAB18189).
    Peptide "+" means "peptide blocking".
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4286
  • Gene Name:
  • MITF
  • Gene Alias:
  • MI,WS2A,bHLHe32
  • Gene Description:
  • microphthalmia-associated transcription factor
  • Gene Summary:
  • This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000195123,OTTHUMP00000195140,homolog of mouse microphthalmia
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