LMNA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LMNA.
Immunogen
A synthetic peptide corresponding to human LMNA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to LMNA.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, using LMNA polyclonal antibody (Cat # PAB18184).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LMNA polyclonal antibody (Cat # PAB18184).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using LMNA polyclonal antibody (Cat # PAB18184).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — LMNA
Entrez GeneID
4000Protein Accession#
P02545Gene Name
LMNA
Gene Alias
CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, PRO1
Gene Description
lamin A/C
Gene Ontology
HyperlinkGene Summary
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq
Other Designations
70 kDa lamin|OTTHUMP00000015843|OTTHUMP00000015848
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Interactome
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Pathway
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Disease
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Publication Reference
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Lamins A and C but not lamin B1 regulate nuclear mechanics.
Lammerding J, Fong LG, Ji JY, Reue K, Stewart CL, Young SG, Lee RT.
The Journal of Biological Chemistry 2006 Sep; 281(35):25768.
Application:IF, WB, Mouse, Mouse embryo fibroblasts.
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Hutchinson-Gilford progeria mutant lamin A primarily targets human vascular cells as detected by an anti-Lamin A G608G antibody.
McClintock D, Gordon LB, Djabali K.
PNAS 2006 Feb; 103(7):2154.
Application:IF, WB, Human, Dermal fibroblasts.
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Lamin A/C expression is a marker of mouse and human embryonic stem cell differentiation.
Constantinescu D, Gray HL, Sammak PJ, Schatten GP, Csoka AB.
Stem Cells 2006 Jan; 24(1):177.
Application:ICC, IF, Human, Human embryonic stem cells, Mouse embryonic stem cells.
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Lamins A and C but not lamin B1 regulate nuclear mechanics.
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