FOXO1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FOXO1.
Immunogen
A synthetic peptide corresponding to human FOXO1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to FOXO1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with Serum (20 %, 15 mins), using FOXO1 polyclonal antibody (Cat # PAB18177).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOXO1 polyclonal antibody (Cat # PAB18177).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using FOXO1 polyclonal antibody (Cat # PAB18177).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — FOXO1
Entrez GeneID
2308Protein Accession#
Q12778Gene Name
FOXO1
Gene Alias
FKH1, FKHR, FOXO1A
Gene Description
forkhead box O1
Gene Ontology
HyperlinkGene Summary
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018301|forkhead box O1A (rhabdomyosarcoma)|forkhead homolog in rhabdomyosarcoma|forkhead, Drosophila, homolog of, in rhabdomyosarcoma
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Interactome
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Pathway
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Disease
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Publication Reference
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FOXO1A is a candidate for the 13q14 tumor suppressor gene inhibiting androgen receptor signaling in prostate cancer.
Dong XY, Chen C, Sun X, Guo P, Vessella RL, Wang RX, Chung LW, Zhou W, Dong JT.
Cancer Research 2006 Jul; 66(14):6998.
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Forkhead transcription factor FOXO1A is critical for induction of human decidualization.
Grinius L, Kessler C, Schroeder J, Handwerger S.
The Journal of Endocrinology 2006 Apr; 189(1):179.
Application:WB-Tr, Human, Human decidual fibroblasts.
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FOXO1a acts as a selective tumor suppressor in alveolar rhabdomyosarcoma.
Bois PR, Izeradjene K, Houghton PJ, Cleveland JL, Houghton JA, Grosveld GC.
The Journal of Cell Biology 2005 Sep; 170(6):903.
Application:IF, WB, Human, Mouse, C2C12 cells, Human habdomyosarcoma, Rh30 cells.
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FOXO1A is a candidate for the 13q14 tumor suppressor gene inhibiting androgen receptor signaling in prostate cancer.
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