ETS1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with Serum (20 %, 15 mins), using ETS1 polyclonal antibody (Cat # PAB18172).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ETS1 polyclonal antibody (Cat # PAB18172).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using ETS1 polyclonal antibody (Cat # PAB18172).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ETS1.
Immunogen
A synthetic peptide corresponding to human ETS1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to ETS1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with Serum (20 %, 15 mins), using ETS1 polyclonal antibody (Cat # PAB18172).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ETS1 polyclonal antibody (Cat # PAB18172).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using ETS1 polyclonal antibody (Cat # PAB18172).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — ETS1
Entrez GeneID
2113Protein Accession#
P14921Gene Name
ETS1
Gene Alias
ETS-1, EWSR2, FLJ10768
Gene Description
v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
Omim ID
164720Gene Ontology
HyperlinkGene Summary
ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM
Other Designations
Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1|ets protein|v-ets avian erythroblastosis virus E2 oncogene homolog 1|v-ets avian erythroblastosis virus E26 oncogene homolog 1|v-ets erythroblastosis virus E26 oncogene homolog 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Thymomegaly, microsplenia, and defective homeostatic proliferation of peripheral lymphocytes in p51-Ets1 isoform-specific null mice.
Higuchi T, Bartel FO, Masuya M, Deguchi T, Henderson KW, Li R, Muise-Helmericks RC, Kern MJ, Watson DK, Spyropoulos DD.
Molecular and Cellular Biology 2007 May; 27(9):3353.
Application：WB-Ti, Mouse, Mouse spleen, thymus.
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Thymomegaly, microsplenia, and defective homeostatic proliferation of peripheral lymphocytes in p51-Ets1 isoform-specific null mice.
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