CHEK2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CHEK2.
Immunogen
A synthetic peptide corresponding to human CHEK2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to CHEK2.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells, using CHEK2 polyclonal antibody (Cat # PAB18147).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CHEK2 polyclonal antibody (Cat # PAB18147).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CHEK2
Entrez GeneID
11200Protein Accession#
O96017Gene Name
CHEK2
Gene Alias
CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53
Gene Description
CHK2 checkpoint homolog (S. pombe)
Gene Ontology
HyperlinkGene Summary
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000028871|checkpoint-like protein CHK2|protein kinase CHK2|serine/threonine-protein kinase CHK2
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Interactome
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Pathway
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Disease
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Publication Reference
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Promyelocytic leukemia activates Chk2 by mediating Chk2 autophosphorylation.
Yang S, Jeong JH, Brown AL, Lee CH, Pandolfi PP, Chung JH, Kim MK.
The Journal of Biological Chemistry 2006 Sep; 281(36):26645.
Application:IF, IP, WB, Human, Mouse, HEK 293T cells, MEFs, Mouse splenocytes, NB4 cells.
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Regulation of the Chk2 protein kinase by oligomerization-mediated cis- and trans-phosphorylation.
Schwarz JK, Lovly CM, Piwnica-Worms H.
Molecular Cancer Research 2003 Jun; 1(8):598.
Application:WB-Ce, WB-Tr, Human, HeLa, AT cells.
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Chk2 is a tumor suppressor that regulates apoptosis in both an ataxia telangiectasia mutated (ATM)-dependent and an ATM-independent manner.
Hirao A, Cheung A, Duncan G, Girard PM, Elia AJ, Wakeham A, Okada H, Sarkissian T, Wong JA, Sakai T, De Stanchina E, Bristow RG, Suda T, Lowe SW, Jeggo PA, Elledge SJ, Mak TW.
Molecular and Cellular Biology 2002 Sep; 22(18):6521.
Application:WB-Ce, WB-Ti, WB-Tr, Mouse, Brain,Thymus, Spleen, Liver, Colon, Testis, Thymocytes, ES cells.
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Promyelocytic leukemia activates Chk2 by mediating Chk2 autophosphorylation.
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