CDC6 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDC6.
Immunogen
A synthetic peptide corresponding to human CDC6.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to CDC6.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HT-29 cells, using CDC6 polyclonal antibody (Cat # PAB18146).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CDC6 polyclonal antibody (Cat # PAB18146).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC6
Entrez GeneID
990Protein Accession#
Q99741Gene Name
CDC6
Gene Alias
CDC18L, HsCDC18, HsCDC6
Gene Description
cell division cycle 6 homolog (S. cerevisiae)
Omim ID
602627Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq
Other Designations
CDC18 (cell division cycle 18, S.pombe, homolog)-like|CDC6 cell division cycle 6 homolog|CDC6-related protein|cell division cycle 6 protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage.
Hall JR, Kow E, Nevis KR, Lu CK, Luce KS, Zhong Q, Cook JG.
Molecular Biology of the Cell 2007 Sep; 18(9):3340.
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Identification and characterization of Saccharomyces cerevisiae Cdc6 DNA-binding properties.
Feng L, Wang B, Driscoll B, Jong A.
Molecular Biology of the Cell 2000 May; 11(5):1673.
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The stability of the Cdc6 protein is regulated by cyclin-dependent kinase/cyclin B complexes in Saccharomyces cerevisiae.
Calzada A, Sanchez M, Sanchez E, Bueno A.
The Journal of Biological Chemistry 2000 Mar; 275(13):9734.
Application:WB-Ce, WB-Re, Yeast, Recombinant protein, Yeast cells.
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Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage.
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