Eif4ebp1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Eif4ebp1.
Immunogen
A synthetic peptide corresponding to mouse Eif4ebp1.
Host
Rabbit
Reactivity
Mouse, Rat
Specificity
This antibody is specific to Eif4ebp1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, treated with EGF (200 ng/mL, 30 mins), using EIF4EBP1 polyclonal antibody (Cat # PAB18143).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EIF4EBP1 polyclonal antibody (Cat # PAB18143).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — Eif4ebp1
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Publication Reference
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Phosphorylation of 4E-BP1 is mediated by the p38/MSK1 pathway in response to UVB irradiation.
Liu G, Zhang Y, Bode AM, Ma WY, Dong Z.
The Journal of Biological Chemistry 2002 Mar; 277(11):8810.
Application:IP, WB, Mouse, JB6 Cl 41 cells.
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Rapamycin-insensitive regulation of 4e-BP1 in regenerating rat liver.
Jiang YP, Ballou LM, Lin RZ.
The Journal of Biological Chemistry 2001 Apr; 276(14):10943.
Application:WB-Ti, Rat, Rat livers.
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Phosphorylation of 4E-BP1 is mediated by the p38/MSK1 pathway in response to UVB irradiation.
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