SRF polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125 ng/mL, 30 mins), using SRF polyclonal antibody (Cat # PAB18137).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SRF polyclonal antibody (Cat # PAB18137).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SRF.
Immunogen
A synthetic peptide corresponding to human SRF.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to SRF.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:300-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125 ng/mL, 30 mins), using SRF polyclonal antibody (Cat # PAB18137).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SRF polyclonal antibody (Cat # PAB18137).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — SRF
Entrez GeneID
6722Protein Accession#
P11831Gene Name
SRF
Gene Alias
MCM1
Gene Description
serum response factor (c-fos serum response element-binding transcription factor)
Omim ID
600589Gene Ontology
HyperlinkGene Summary
This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). [provided by RefSeq
Other Designations
OTTHUMP00000039820
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of a new hybrid serum response factor and myocyte enhancer factor 2-binding element in MyoD enhancer required for MyoD expression during myogenesis.
L'honore A, Rana V, Arsic N, Franckhauser C, Lamb NJ, Fernandez A.
Molecular Biology of the Cell 2007 Jun; 18(6):1992.
Application：ChIP, Func, Mouse, C2C12 cells, C2 myoblasts.
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Regulation of Egr-1, SRF, and Sp1 mRNA expression in contracting skeletal muscle cells.
Irrcher I, Hood DA.
Journal of Applied Physiology 2004 Dec; 97(6):2207.
Application：Func, Mouse, C2C12 cells.
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Identification of a new hybrid serum response factor and myocyte enhancer factor 2-binding element in MyoD enhancer required for MyoD expression during myogenesis.
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