RAD17 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RAD17.
Immunogen
A synthetic peptide corresponding to human RAD17.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to RAD17.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A-549 cells, using RAD17 polyclonal antibody (Cat # PAB18134).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using RAD17 polyclonal antibody (Cat # PAB18134).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using RAD17 polyclonal antibody (Cat # PAB18134).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAD17
Entrez GeneID
5884Protein Accession#
O75943Gene Name
RAD17
Gene Alias
CCYC, FLJ41520, HRAD17, R24L, RAD17SP, RAD24
Gene Description
RAD17 homolog (S. pombe)
Omim ID
603139Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000125189|OTTHUMP00000125190|OTTHUMP00000125192|OTTHUMP00000125193|OTTHUMP00000125194|RAD1 homolog|RAD17 homolog|RF-C activator 1 homolog|Rad17-like protein|cell cycle checkpoint protein (RAD17)
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Interactome
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Disease
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Publication Reference
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Chromatin association of rad17 is required for an ataxia telangiectasia and rad-related kinase-mediated S-phase checkpoint in response to low-dose ultraviolet radiation.
Garg R, Callens S, Lim DS, Canman CE, Kastan MB, Xu B.
Molecular Cancer Research 2004 Jun; 2(6):362.
Application:WB-Tr, Human, HEK 293T cells.
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Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatin.
Zou L, Cortez D, Elledge SJ.
Genes & Development 2002 Jan; 16(2):198.
Application:WB-Tr, Human, HCT-116, HEK 293T, HeLa cells.
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Phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G(1)/S checkpoint activation in response to DNA damage.
Post S, Weng YC, Cimprich K, Chen LB, Xu Y, Lee EY.
PNAS 2001 Nov; 98(23):13102.
Application:WB-Ti, WB-Tr, Human, Mouse, HEK 293 cells, Mouse lungs.
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Chromatin association of rad17 is required for an ataxia telangiectasia and rad-related kinase-mediated S-phase checkpoint in response to low-dose ultraviolet radiation.
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