POLR2A polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells treated with EGF (200 ng/mL, 30 min), using POLR2A polyclonal antibody (Cat # PAB18131).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A polyclonal antibody (Cat # PAB18131).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, treated with PMA (125 ng/mL, 30 mins), using POLR2A polyclonal antibody (Cat # PAB18131).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of POLR2A.
Immunogen
A synthetic peptide corresponding to human POLR2A.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to POLR2A.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells treated with EGF (200 ng/mL, 30 min), using POLR2A polyclonal antibody (Cat # PAB18131).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A polyclonal antibody (Cat # PAB18131).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, treated with PMA (125 ng/mL, 30 mins), using POLR2A polyclonal antibody (Cat # PAB18131).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — POLR2A
Entrez GeneID
5430Protein Accession#
P24928Gene Name
POLR2A
Gene Alias
MGC75453, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
Gene Description
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Omim ID
180660Gene Ontology
HyperlinkGene Summary
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq
Other Designations
DNA directed RNA polymerase II polypeptide A|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|polymerase (RNA) II (DNA directed) polypeptide A (220kD)
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Interactome
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Pathway
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Disease
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Publication Reference
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Evaluation of locked nucleic acid-modified small interfering RNA in vitro and in vivo.
Mook OR, Baas F, de Wissel MB, Fluiter K.
Molecular Cancer Therapeutics 2007 Mar; 6(3):833.
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Functional genomic analysis of herpes simplex virus type 1 counteraction of the host innate response.
Pasieka TJ, Baas T, Carter VS, Proll SC, Katze MG, Leib DA.
Journal of Virology 2006 Aug; 80(15):7600.
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Functional and genomic changes in the mouse ocular motor system in response to light deprivation from birth.
McMullen CA, Andrade FH, Stahl JS.
Journal of Neuroscience 2004 Jan; 24(1):161.
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Evaluation of locked nucleic acid-modified small interfering RNA in vitro and in vivo.
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