KSR1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KSR1.
Immunogen
A synthetic peptide corresponding to human KSR1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to KSR1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells treated with PDGF (50 ng/mL, 20 min), using KSR1 polyclonal antibody (Cat # PAB18125).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using KSR1 polyclonal antibody (Cat # PAB18125).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using KSR1 polyclonal antibody (Cat # PAB18125).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — KSR1
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Interactome
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Disease
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Publication Reference
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KSR is a scaffold required for activation of the ERK/MAPK module.
Roy F, Laberge G, Douziech M, Ferland-McCollough D, Therrien M.
Genes & Development 2002 Feb; 16(4):427.
Application:WB-Tr, Drosophila, S2 cells.
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KSR: a MAPK scaffold of the Ras pathway?
Morrison DK.
Journal of Cell Science 2001 May; 114(Pt 9):1609.
Application:WB, Human, Mammalican cells.
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KSR is a scaffold required for activation of the ERK/MAPK module.
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