EEF2K polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EEF2K.
Immunogen
A synthetic peptide corresponding to human EEF2K.
Host
Rabbit
Reactivity
Human, Rat
Specificity
This antibody is specific to EEF2K.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:300-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with serum (10 %, 15 mins), using EEF2K polyclonal antibody (Cat # PAB18123).
Peptide "+" means "peptide blocking".Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — EEF2K
Entrez GeneID
29904Protein Accession#
O00418Gene Name
EEF2K
Gene Alias
HSU93850, MGC45041, eEF-2K
Gene Description
eukaryotic elongation factor-2 kinase
Omim ID
606968Gene Ontology
HyperlinkGene Summary
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq
Other Designations
calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase|calmodulin-dependent protein kinase III|eEF-2 kinase|elongation factor-2 kinase
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Interactome
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Disease
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Publication Reference
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Alcohol regulates eukaryotic elongation factor 2 phosphorylation via an AMP-activated protein kinase-dependent mechanism in C2C12 skeletal myocytes.
Hong-Brown LQ, Brown CR, Huber DS, Lang CH.
Journal of Biological Chemistry 2007 Feb; 282(6):3702.
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Farnesyltransferase inhibitor SCH66336 induces rapid phosphorylation of eukaryotic translation elongation factor 2 in head and neck squamous cell carcinoma cells.
Ren H, Tai SK, Khuri F, Chu Z, Mao L.
Cancer Research 2005 Jul; 65(13):5841.
Application:WB, Human, UMSCC38 cells.
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Myocardial ischemia and increased heart work modulate the phosphorylation state of eukaryotic elongation factor-2.
Horman S, Beauloye C, Vertommen D, Vanoverschelde JL, Hue L, Rider MH.
The Journal of Biological Chemistry 2003 Oct; 278(43):41970.
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Alcohol regulates eukaryotic elongation factor 2 phosphorylation via an AMP-activated protein kinase-dependent mechanism in C2C12 skeletal myocytes.
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