CRK polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, using CRK polyclonal antibody (Cat # PAB18119).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CRK polyclonal antibody (Cat # PAB18119).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using CRK polyclonal antibody (Cat # PAB18119).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CRK.
Immunogen
A synthetic peptide corresponding to human CRK.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to CRK.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, using CRK polyclonal antibody (Cat # PAB18119).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CRK polyclonal antibody (Cat # PAB18119).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using CRK polyclonal antibody (Cat # PAB18119).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CRK
Entrez GeneID
1398Protein Accession#
P46108Gene Name
CRK
Gene Alias
CRKII
Gene Description
v-crk sarcoma virus CT10 oncogene homolog (avian)
Omim ID
164762Gene Ontology
HyperlinkGene Summary
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq
Other Designations
avian sarcoma virus CT10 (v-crk) oncogene homolog|v-crk avian sarcoma virus CT10 oncogene homolog|v-crk sarcoma virus CT10 oncogene homolog
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Interactome
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Pathway
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Publication Reference
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Anabolic effects of a G protein-coupled receptor kinase inhibitor expressed in osteoblasts.
Spurney RF, Flannery PJ, Garner SC, Athirakul K, Liu S, Guilak F, Quarles LD.
The Journal of Clinical Investigation 2002 May; 109(10):1361.
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Isolation of Trypanosoma brucei CYC2 and CYC3 cyclin genes by rescue of a yeast G(1) cyclin mutant. Functional characterization of CYC2.
Van Hellemond JJ, Neuville P, Schwarz RT, Matthews KR, Mottram JC.
The Journal of Biological Chemistry 2000 Mar; 275(12):8315.
Application：WB-Tr, S. cerevisiae, Strain L40.
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Anabolic effects of a G protein-coupled receptor kinase inhibitor expressed in osteoblasts.
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