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Last updated: 2016/12/4
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PLA2G4A polyclonal antibody

  • Catalog # : PAB18118
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PLA2G4A.
  • Immunogen:
  • A synthetic peptide corresponding to human PLA2G4A.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to PLA2G4A.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Concentration:
  • 1 mg/mL
  • Recommend Usage:
  • Western Blot (1:500-1:1000)
    Immunohistochemistry (1:50-1:100)
    Immunofluorescence (1:500-1:1000)
    ELISA (1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from HeLa cells, treated with TNF-a (20 ng/mL, 30 mins), using PLA2G4A polyclonal antibody (Cat # PAB18118).
    Peptide "+" means "peptide blocking".
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PLA2G4A polyclonal antibody (Cat # PAB18118).
    Peptide "+" means "peptide blocking".
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HUVEC cells, using PLA2G4A polyclonal antibody (Cat # PAB18118).
    Peptide "+" means "peptide blocking".
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5321
  • Gene Name:
  • PLA2G4A
  • Gene Alias:
  • MGC126350,PLA2G4,cPLA2-alpha
  • Gene Description:
  • phospholipase A2, group IVA (cytosolic, calcium-dependent)
  • Gene Summary:
  • This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000033543,calcium-dependent phospholipid-binding protein,cytosolic phospholipase A2, group IVA,lysophospholipase,phosphatidylcholine 2-acylhydrolase
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