PLA2G4A polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PLA2G4A.
Immunogen
A synthetic peptide corresponding to human PLA2G4A.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PLA2G4A.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with TNF-a (20 ng/mL, 30 mins), using PLA2G4A polyclonal antibody (Cat # PAB18118).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PLA2G4A polyclonal antibody (Cat # PAB18118).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using PLA2G4A polyclonal antibody (Cat # PAB18118).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PLA2G4A
Entrez GeneID
5321Protein Accession#
P47712Gene Name
PLA2G4A
Gene Alias
MGC126350, PLA2G4, cPLA2-alpha
Gene Description
phospholipase A2, group IVA (cytosolic, calcium-dependent)
Omim ID
600522Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq
Other Designations
OTTHUMP00000033543|calcium-dependent phospholipid-binding protein|cytosolic phospholipase A2, group IVA|lysophospholipase|phosphatidylcholine 2-acylhydrolase
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Interactome
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Publication Reference
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Identification of intracellular phospholipases A2 in the human eye: involvement in phagocytosis of photoreceptor outer segments.
Kolko M, Wang J, Zhan C, Poulsen KA, Prause JU, Nissen MH, Heegaard S, Bazan NG.
Investigative Ophthalmology & Visual Science 2007 Mar; 48(3):1401.
Application:IHC-P, WB, Human, ARPE-19 cells, Human eyes.
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An ovulatory gonadotropin stimulus increases cytosolic phospholipase A2 expression and activity in granulosa cells of primate periovulatory follicles.
Duffy DM, Seachord CL, Dozier BL.
The Journal of clinical endocrinology and metabolism 2005 Oct; 90(10):5858.
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Activation of BK channels in GH3 cells by a c-PLA2-dependent G-protein signaling pathway.
Denson DD, Li J, Wang X, Eaton DC.
Journal of Neurophysiology 2005 Jun; 93(6):3146.
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Identification of intracellular phospholipases A2 in the human eye: involvement in phagocytosis of photoreceptor outer segments.
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