LIMK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, using LIMK1 polyclonal antibody (Cat # PAB18112).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 polyclonal antibody (Cat # PAB18112).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LIMK1.
Immunogen
A synthetic peptide corresponding to human LIMK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to LIMK1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, using LIMK1 polyclonal antibody (Cat # PAB18112).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 polyclonal antibody (Cat # PAB18112).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — LIMK1
Entrez GeneID
3984Protein Accession#
P53667Gene Name
LIMK1
Gene Alias
LIMK
Gene Description
LIM domain kinase 1
Omim ID
601329Gene Ontology
HyperlinkGene Summary
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. [provided by RefSeq
Other Designations
LIM motif-containing protein kinase|OTTHUMP00000025066
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Interactome
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Pathway
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Disease
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Publication Reference
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The Rho GTPase effector ROCK regulates cyclin A, cyclin D1, and p27Kip1 levels by distinct mechanisms.
Croft DR, Olson MF.
Molecular and Cellular Biology 2006 Jun; 26(12):4612.
Application：WB-Tr, Mouse, NIH/3T3 cells.
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Par-3 mediates the inhibition of LIM kinase 2 to regulate cofilin phosphorylation and tight junction assembly.
Chen X, Macara IG.
The Journal of Cell Biology 2006 Feb; 172(5):671.
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Actin-myosin-based contraction is responsible for apoptotic nuclear disintegration.
Croft DR, Coleman ML, Li S, Robertson D, Sullivan T, Stewart CL, Olson MF.
Journal of Cell Biology 2005 Jan; 168(2):245.
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The Rho GTPase effector ROCK regulates cyclin A, cyclin D1, and p27Kip1 levels by distinct mechanisms.
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