KRT18 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with UV (5 min), using KRT18 polyclonal antibody (Cat # PAB18098).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using KRT18 polyclonal antibody (Cat # PAB18098).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using KRT18 polyclonal antibody (Cat # PAB18098).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KRT18.
Immunogen
A synthetic peptide corresponding to human KRT18.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to KRT18.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with UV (5 min), using KRT18 polyclonal antibody (Cat # PAB18098).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using KRT18 polyclonal antibody (Cat # PAB18098).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using KRT18 polyclonal antibody (Cat # PAB18098).
Peptide "+" means "peptide blocking".Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — KRT18
Entrez GeneID
3875Protein Accession#
P05783Gene Name
KRT18
Gene Alias
CYK18, K18
Gene Description
keratin 18
Gene Ontology
HyperlinkGene Summary
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
cell proliferation-inducing protein 46|cytokeratin 18
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Interactome
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Pathway
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Disease
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Publication Reference
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Cell-specific expression of functional glucose transporter 8 in mammary gland.
Lorena Mardones, Katia Muñoz, Marcelo Villagrán.
Biochemical and Biophysical Research Communications 2021 Aug; 567:125.
Application：IF, IHC-P, Human, Mouse, 1-7HB2 cells, Mouse mammary glands.
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Keratins turn over by ubiquitination in a phosphorylation-modulated fashion.
Ku NO, Omary MB.
The Journal of Cell Biology 2000 May; 149(3):547.
Application：WB-Ce, WB-Tr, Human, Mouse, HT-29, BHK cells.
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Cell-specific expression of functional glucose transporter 8 in mammary gland.
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