NFKBIE polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NFKBIE.
Immunogen
A synthetic peptide corresponding to human NFKBIE.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to NFKBIE.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with TNF-a (20 ng/mL 5 min), using NFKBIE polyclonal antibody (Cat # PAB18097).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFKBIE polyclonal antibody (Cat # PAB18097).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using NFKBIE polyclonal antibody (Cat # PAB18097).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFKBIE
Entrez GeneID
4794Protein Accession#
O00221Gene Name
NFKBIE
Gene Alias
IKBE
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon
Omim ID
604548Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT (see STAT6, MIM 601512), AP1 (JUN, MIM 165160), and NFAT (see NFATC1, MIM 600489).[supplied by OMIM
Other Designations
OTTHUMP00000016522
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of NAP1, a regulatory subunit of IkappaB kinase-related kinases that potentiates NF-kappaB signaling.
Fujita F, Taniguchi Y, Kato T, Narita Y, Furuya A, Ogawa T, Sakurai H, Joh T, Itoh M, Delhase M, Karin M, Nakanishi M.
Molecular and Cellular Biology 2003 Nov; 23(21):7780.
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NFkappaB and caspase-3 activity in apoptotic hepatocytes of galactosamine-sensitized mice treated with TNFalpha.
Tapalaga D, Tiegs G, Angermuller S.
The Journal of Histochemistry and Cytochemistry 2002 Dec; 50(12):1599.
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Interaction between hnRNPA1 and IkappaBalpha is required for maximal activation of NF-kappaB-dependent transcription.
Hay DC, Kemp GD, Dargemont C, Hay RT.
Molecular and Cellular Biology 2001 May; 21(10):3482.
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Identification of NAP1, a regulatory subunit of IkappaB kinase-related kinases that potentiates NF-kappaB signaling.
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