PRKAA1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKAA1.
Immunogen
A synthetic peptide corresponding to human PRKAA1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PRKAA1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells treated with Adriamycin (0.5 ng/mL, 24 hours) using PRKAA1 polyclonal antibody (Cat # PAB18089).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PRKAA1 polyclonal antibody (Cat # PAB18089).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKAA1
Entrez GeneID
5562Protein Accession#
Q13131Gene Name
PRKAA1
Gene Alias
AMPK, AMPKa1, MGC33776, MGC57364
Gene Description
protein kinase, AMP-activated, alpha 1 catalytic subunit
Omim ID
602739Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, catalytic alpha-1 chain|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Genetic model for the chronic activation of skeletal muscle AMP-activated protein kinase leads to glycogen accumulation.
Barre L, Richardson C, Hirshman MF, Brozinick J, Fiering S, Kemp BE, Goodyear LJ, Witters LA.
American Journal of Physiology. Endocrinology and Metabolism 2007 Mar; 292(3):E802.
Application:WB, Mouse, Mouse gastrocnemius muscles.
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Adenosine 5'-monophosphate-activated protein kinase regulates progesterone secretion in rat granulosa cells.
Tosca L, Froment P, Solnais P, Ferre P, Foufelle F, Dupont J.
Endocrinology 2005 Oct; 146(10):4500.
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Glycogen debranching enzyme association with beta-subunit regulates AMP-activated protein kinase activity.
Sakoda H, Fujishiro M, Fujio J, Shojima N, Ogihara T, Kushiyama A, Fukushima Y, Anai M, Ono H, Kikuchi M, Horike N, Viana AY, Uchijima Y, Kurihara H, Asano T.
American Journal of Physiology. Endocrinology and Metabolism 2005 Sep; 289(3):E474.
Application:IP, WB-Tr, Monkey, COS-7 cells.
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Genetic model for the chronic activation of skeletal muscle AMP-activated protein kinase leads to glycogen accumulation.
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