YWHAZ polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells treated with Forskolin (40 nM, 30 min) using YWHAZ polyclonal antibody (Cat # PAB18088).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using YWHAZ polyclonal antibody (Cat # PAB18088).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, treated with PMA (125 ng/mL, 30 mins), using YWHAZ polyclonal antibody (Cat # PAB18088).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of YWHAZ.
Immunogen
A synthetic peptide corresponding to human YWHAZ.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to YWHAZ.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells treated with Forskolin (40 nM, 30 min) using YWHAZ polyclonal antibody (Cat # PAB18088).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using YWHAZ polyclonal antibody (Cat # PAB18088).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, treated with PMA (125 ng/mL, 30 mins), using YWHAZ polyclonal antibody (Cat # PAB18088).
Peptide "+" means "peptide blocking".Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — YWHAZ
Entrez GeneID
7534Protein Accession#
P63104Gene Name
YWHAZ
Gene Alias
KCIP-1, MGC111427, MGC126532, MGC138156
Gene Description
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Omim ID
601288Gene Ontology
HyperlinkGene Summary
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq
Other Designations
14-3-3 protein/cytosolic phospholipase A2|14-3-3 zeta|OTTHUMP00000165851|OTTHUMP00000165852|OTTHUMP00000165854|OTTHUMP00000165858|OTTHUMP00000165859|OTTHUMP00000165860|phospholipase A2|protein kinase C inhibitor protein-1|tyrosine 3/tryptophan 5 -monooxyg
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Interactome
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Pathway
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Disease
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Publication Reference
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An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.
Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY.
Molecular & Cellular Proteomics 2013 May; 12(5):1335.
Application：Profiling, Human, Huh7 cells, Mahlavu cells.
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Dynamic interactions between 14-3-3 proteins and phosphoproteins regulate diverse cellular processes.
Mackintosh C.
The Biochemical Journal 2004 Jul; 381(Pt 2):329.
Application：IHC, WB, Human, Mammalian cells.
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Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding.
Powell DW, Rane MJ, Joughin BA, Kalmukova R, Hong JH, Tidor B, Dean WL, Pierce WM, Klein JB, Yaffe MB, McLeish KR.
Molecular and Cellular Biology 2003 Aug; 23(15):5376.
Application：WB, Human, Neutrophils, Recombinant protein.
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Significance of 14-3-3 self-dimerization for phosphorylation-dependent target binding.
Shen YH, Godlewski J, Bronisz A, Zhu J, Comb MJ, Avruch J, Tzivion G.
Molecular and Cellular Biology 2003 Nov; 14(11):4721.
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An analysis of protein-protein interactions in cross-talk pathways reveals CRKL as a novel prognostic marker in hepatocellular carcinoma.
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