RNF7 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
RNF7 polyclonal antibody (Cat # PAB17858) (1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RNF7.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human RNF7.
Sequence
DACLRCQAENKQE
Host
Goat
Theoretical MW (kDa)
12.7, 9.64
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RNF7 polyclonal antibody (Cat # PAB17858) (1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF7
Entrez GeneID
9616Protein Accession#
NP_055060.1Gene Name
RNF7
Gene Alias
CKBBP1, ROC2, SAG
Gene Description
ring finger protein 7
Omim ID
603863Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000180645|regulator of cullins 2|sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2|zinc RING finger protein SAG
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Interactome
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Pathway
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