SMC5 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SMC5.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human SMC5.
Sequence
C-TSKHLEHKDNELRQ
Host
Goat
Theoretical MW (kDa)
129
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Immunofluorescence (10 ug/mL)
Flow Cytometry (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
SMC5 polyclonal antibody (Cat # PAB17817) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing speckled nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
SMC5 polyclonal antibody (Cat # PAB17817) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — SMC5
Entrez GeneID
23137Protein Accession#
NP_055925.2Gene Name
SMC5
Gene Alias
KIAA0594, SMC5L1
Gene Description
structural maintenance of chromosomes 5
Omim ID
609386Gene Ontology
HyperlinkOther Designations
OTTHUMP00000063367|SMC5 protein|SMC5 structural maintenance of chromosomes 5-like 1
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