NOX5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NOX5.
Immunogen
A synthetic peptide corresponding to internal of human NOX5.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total NOX5 protein
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells, using NOX5 polyclonal antibody (Cat # PAB17793).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — NOX5
Entrez GeneID
79400Protein Accession#
Q96PH1Gene Name
NOX5
Gene Alias
MGC149776, MGC149777, NOX5A, NOX5B
Gene Description
NADPH oxidase, EF-hand calcium binding domain 5
Omim ID
606572Gene Ontology
HyperlinkGene Summary
NOX5 is a novel NADPH oxidase that generates superoxide and functions as a H+ channel in a Ca(2+)-dependent manner.[supplied by OMIM
Other Designations
NADPH oxidase, EF hand calcium-binding domain 5|NOX5 variant lacking EF hands|OTTHUMP00000164443
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Interactome
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Disease
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Publication Reference
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Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells.
Jie Chen, Yan Wang, Weimin Zhang, Di Zhao, Lingyuan Zhang, Jiawen Fan, Jinting Li, Qimin Zhan.
Signal Transduction and Targeted Therapy 2020 Aug; 5(1):139.
Application:IHC-P, WB-Ce, WB-Ti, WB-Tr, Human, Human esophageal epithelial cells, Human esophageal squamous cell carcinoma tumors, KYSE30 cells, KYSE410 cells.
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Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells.
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