ME3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells (Lane 1) and RAW 264.7 cells (Lane 2 and lane 3), using ME3 polyclonal antibody (Cat # PAB17792).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ME3.
Immunogen
A synthetic peptide corresponding to internal of human ME3.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total ME3 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells (Lane 1) and RAW 264.7 cells (Lane 2 and lane 3), using ME3 polyclonal antibody (Cat # PAB17792).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — ME3
Entrez GeneID
10873Protein Accession#
Q16798Gene Name
ME3
Gene Alias
FLJ34862
Gene Description
malic enzyme 3, NADP(+)-dependent, mitochondrial
Omim ID
604626Gene Ontology
HyperlinkGene Summary
Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq
Other Designations
NADP-ME|malate dehydrogenase|malic enzyme, NADP+-dependent, mitochondrial|mitochondrial NADP(+)-dependent malic enzyme 3|pyruvic-malic carboxylase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Metabolic pathways
- Pyruvate metabolism
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Disease
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