ME2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ME2.
Immunogen
A synthetic peptide corresponding to internal of human ME2.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total ME2 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from LoVo cells (Lane 1) and RAW 264.7 cells (Lane 2 and lane 3), using ME2 polyclonal antibody (Cat # PAB17785).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ME2 polyclonal antibody (Cat # PAB17785).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — ME2
Entrez GeneID
4200Protein Accession#
P23368Gene Name
ME2
Gene Alias
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Gene Description
malic enzyme 2, NAD(+)-dependent, mitochondrial
Gene Ontology
HyperlinkGene Summary
This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. [provided by RefSeq
Other Designations
Malic enzyme, mitochondrial|malate dehydrogenase|malic enzyme 2, mitochondrial|pyruvic-malic carboxylase
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Interactome
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Pathway
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Disease
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