MRPS22 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, using MRPS22 polyclonal antibody (Cat # PAB17765).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of A-549 cells, using MRPS22 polyclonal antibody (Cat # PAB17765).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MRPS22.
Immunogen
A synthetic peptide corresponding to internal of human MRPS22.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total MRPS22 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, using MRPS22 polyclonal antibody (Cat # PAB17765).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using MRPS22 polyclonal antibody (Cat # PAB17765).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MRPS22
Entrez GeneID
56945Protein Accession#
P82650Gene Name
MRPS22
Gene Alias
C3orf5, COXPD5, GIBT, GK002, MRP-S22, RPMS22
Gene Description
mitochondrial ribosomal protein S22
Omim ID
605810Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to this gene is found on chromosome Xq. [provided by RefSeq
Other Designations
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