MRPS21 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, using MRPS21 polyclonal antibody (Cat # PAB17764).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using MRPS21 polyclonal antibody (Cat # PAB17764).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of MCF-7 cells, using MRPS21 polyclonal antibody (Cat # PAB17764).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MRPS21.
Immunogen
A synthetic peptide corresponding to internal of human MRPS21.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total MRPS21 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, using MRPS21 polyclonal antibody (Cat # PAB17764).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using MRPS21 polyclonal antibody (Cat # PAB17764).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of MCF-7 cells, using MRPS21 polyclonal antibody (Cat # PAB17764).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MRPS21
Entrez GeneID
54460Protein Accession#
P82921Gene Name
MRPS21
Gene Alias
MDS016, MRP-S21, RPMS21
Gene Description
mitochondrial ribosomal protein S21
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000014527|OTTHUMP00000014921|mitochondrial 28S ribosomal protein S21
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