UBE2B polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
The UBE2B polyclonal antibody (Cat # PAB1776) is used in Western blot to detect UBE2B in A-375 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2B polyclonal antibody (Cat # PAB1776) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBE2B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBE2B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
The UBE2B polyclonal antibody (Cat # PAB1776) is used in Western blot to detect UBE2B in A-375 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2B polyclonal antibody (Cat # PAB1776) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — UBE2B
Entrez GeneID
7320Gene Name
UBE2B
Gene Alias
E2-17kDa, HHR6B, HR6B, RAD6B, UBC2
Gene Description
ubiquitin-conjugating enzyme E2B (RAD6 homolog)
Omim ID
179095Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq
Other Designations
E2 protein|ubiquitin carrier protein B|ubiquitin-conjugating enzyme E2B|ubiquitin-protein ligase B
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31.
Koken MH, Smit EM, Jaspers-Dekker I, Oostra BA, Hagemeijer A, Bootsma D, Hoeijmakers JH.
Genomics 1992 Mar; 12(3):447.
-
Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6.
Koken MH, Reynolds P, Jaspers-Dekker I, Prakash L, Prakash S, Bootsma D, Hoeijmakers JH.
PNAS 1991 Oct; 88(20):8865.
-
The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6.
Schneider R, Eckerskorn C, Lottspeich F, Schweiger M.
The EMBO Journal 1990 May; 9(5):1431.
-
Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com