UBA1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The UBA1 polyclonal antibody (Cat # PAB1772) is used in Western blot to detect UBA1 in HL-60 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBA1 polyclonal antibody (Cat # PAB1772) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with UBA1 polyclonal antibody (Cat # PAB1772) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBA1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBA1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The UBA1 polyclonal antibody (Cat # PAB1772) is used in Western blot to detect UBA1 in HL-60 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBA1 polyclonal antibody (Cat # PAB1772) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with UBA1 polyclonal antibody (Cat # PAB1772) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — UBA1
Entrez GeneID
7317Protein Accession#
UBA1_HUMANGene Name
UBA1
Gene Alias
A1S9, A1S9T, A1ST, AMCX1, GXP1, MGC4781, SMAX2, UBA1A, UBE1, UBE1X
Gene Description
ubiquitin-like modifier activating enzyme 1
Omim ID
314370Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq
Other Designations
A1S9T and BN75 temperature sensitivity complementing|OTTHUMP00000023197|OTTHUMP00000023198|UBA1, ubiquitin-activating enzyme E1 homolog A|ubiquitin-activating enzyme E1
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Interactome
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Pathway
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Disease
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Publication Reference
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Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1.
Ayusawa D, Kaneda S, Itoh Y, Yasuda H, Murakami Y, Sugasawa K, Hanaoka F, Seno T.
Cell Structure and Function 1992 Apr; 17(2):113.
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Human ubiquitin-activating enzyme (E1): compensation for heat-labile mouse E1 and its gene localization on the X chromosome.
Kudo M, Sugasawa K, Hori T, Enomoto T, Hanaoka F, Ui M.
Experimental Cell Research 1991 Jan; 192(1):110.
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Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.
Handley PM, Mueckler M, Siegel NR, Ciechanover A, Schwartz AL.
PNAS 1991 Jan; 88(1):258.
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Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1.
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