EIF3D polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, using EIF3D polyclonal antibody (Cat # PAB17717).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using EIF3D polyclonal antibody (Cat # PAB17717).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF3D.
Immunogen
A synthetic peptide corresponding to internal of human EIF3D.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total EIF3D protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, using EIF3D polyclonal antibody (Cat # PAB17717).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using EIF3D polyclonal antibody (Cat # PAB17717).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF3D
Entrez GeneID
8664Protein Accession#
O15371Gene Name
EIF3D
Gene Alias
EIF3S7, MGC126526, MGC17258, eIF3-p66, eIF3-zeta
Gene Description
eukaryotic translation initiation factor 3, subunit D
Omim ID
603915Gene Ontology
HyperlinkGene Summary
Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq
Other Designations
OTTHUMP00000028733|eukaryotic translation initiation factor 3 subunit D|eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa|translation initiation factor eIF3 p66 subunit
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Interactome
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Disease
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