UBE2E3 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
UBE2E3 polyclonal antibody (Cat # PAB1771) is used in Western blot to detect UBE2E3 in mouse liver tissue lysate.
Western Blot (Cell lysate)
(RIGHT) Western blot analysis of UBE2E3 polyclonal antibody (Cat # PAB1771) in Y-79 cell line lysates (35 ug/lane). UBE2E3 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with UBE2E3 polyclonal antibody (Cat # PAB1771) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBE2E3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human UBE2E3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
UBE2E3 polyclonal antibody (Cat # PAB1771) is used in Western blot to detect UBE2E3 in mouse liver tissue lysate.Western Blot (Cell lysate)
(RIGHT) Western blot analysis of UBE2E3 polyclonal antibody (Cat # PAB1771) in Y-79 cell line lysates (35 ug/lane). UBE2E3 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with UBE2E3 polyclonal antibody (Cat # PAB1771) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — UBE2E3
Entrez GeneID
10477Protein Accession#
Q969T4Gene Name
UBE2E3
Gene Alias
UBCH9, UbcM2
Gene Description
ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
Omim ID
604151Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
ubiquitin carrier protein|ubiquitin-conjugating enzyme E2E 3|ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5)|ubiquitin-protein ligase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9), encoding an N-terminally extended human ubiquitin-conjugating enzyme.
Ito K, Kato S, Matsuda Y, Kimura M, Okano Y.
Cytogenetics and Cell Genetics 1999 Jan; 84(1-2):99.
-
cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9), encoding an N-terminally extended human ubiquitin-conjugating enzyme.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com