PRIM1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRIM1.
Immunogen
A synthetic peptide corresponding to C-terminus of human PRIM1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total PRIM1 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using PRIM1 polyclonal antibody (Cat # PAB17696).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRIM1
Entrez GeneID
5557Protein Accession#
P49642Gene Name
PRIM1
Gene Alias
MGC12308, p49
Gene Description
primase, DNA, polypeptide 1 (49kDa)
Omim ID
176635Gene Ontology
HyperlinkGene Summary
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq
Other Designations
DNA primase 1|DNA primase polypeptide 1|DNA primase small subunit|DNA primase subunit 48|primase p49 subunit|primase polypeptide 1, 49kDa
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