CA5A polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from A-549 cells (Lane 1), LoVo cells (Lane 2) and K-562 cells (Lane 3 and lane 4), using CA5A polyclonal antibody (Cat # PAB17649).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CA5A.
Immunogen
A synthetic peptide corresponding to internal of human CA5A.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total CA5A protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A-549 cells (Lane 1), LoVo cells (Lane 2) and K-562 cells (Lane 3 and lane 4), using CA5A polyclonal antibody (Cat # PAB17649).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CA5A
Entrez GeneID
763Protein Accession#
P35218Gene Name
CA5A
Gene Alias
CA5, CAV, CAVA
Gene Description
carbonic anhydrase VA, mitochondrial
Omim ID
114761Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2. [provided by RefSeq
Other Designations
carbonic anhydrase V, mitochondrial|carbonic dehydratase
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