ARFGEF2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ARFGEF2.
Immunogen
A synthetic peptide corresponding to internal of human ARFGEF2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total ARFGEF2 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A-549 cells, using ARFGEF2 polyclonal antibody (Cat # PAB17638).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using ARFGEF2 polyclonal antibody (Cat # PAB17638).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — ARFGEF2
Entrez GeneID
10564Protein Accession#
Q9Y6D5Gene Name
ARFGEF2
Gene Alias
BIG2, FLJ23723, dJ1164I10.1
Gene Description
ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Gene Ontology
HyperlinkGene Summary
ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq
Other Designations
ADP-ribosylation factor guanine nucleotide-exchange factor 2|OTTHUMP00000031231|brefeldin A-inhibited guanine nucleotide-exchange protein 2
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