ARF4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from LoVo cells (Lane 1), K-562 cells (Lane 2 and lane 4) and HT-29 cells (Lane 3), using ARF4 polyclonal antibody (Cat # PAB17597).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ARF4.
Immunogen
A synthetic peptide corresponding to amino acids 71-120 of human ARF4.
Host
Rabbit
Theoretical MW (kDa)
20
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total ARF4 protein.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
ELISA (1:40000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from LoVo cells (Lane 1), K-562 cells (Lane 2 and lane 4) and HT-29 cells (Lane 3), using ARF4 polyclonal antibody (Cat # PAB17597).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — ARF4
Entrez GeneID
378Protein Accession#
P18085Gene Name
ARF4
Gene Alias
ARF2
Gene Description
ADP-ribosylation factor 4
Omim ID
601177Gene Ontology
HyperlinkGene Summary
This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq
Other Designations
ADP-ribosylation factor 2
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