RPS4X polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO cells (Lane 1) and HeLa cells (Lane 2 and lane 3), using RPS4X polyclonal antibody (Cat # PAB17574).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using RPS4X polyclonal antibody (Cat # PAB17574).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RPS4X.
Immunogen
A synthetic peptide corresponding to internal of human RPS4X.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total RPS4X protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO cells (Lane 1) and HeLa cells (Lane 2 and lane 3), using RPS4X polyclonal antibody (Cat # PAB17574).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using RPS4X polyclonal antibody (Cat # PAB17574).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPS4X
Entrez GeneID
6191Protein Accession#
P62701Gene Name
RPS4X
Gene Alias
CCG2, DXS306, FLJ40595, SCAR, SCR10
Gene Description
ribosomal protein S4, X-linked
Omim ID
312760Gene Ontology
HyperlinkGene Summary
Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
40S ribosomal protein S4, X isoform|OTTHUMP00000023537|cell cycle gene 2|ribosomal protein S4X isoform|single-copy abundant mRNA
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Interactome
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Pathway
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Publication Reference
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The rRNA m6A methyltransferase METTL5 is involved in pluripotency and developmental programs.
Ignatova VV, Stolz P, Kaiser S, Gustafsson TH, Lastres PR, Sanz-Moreno A, Cho YL, Amarie OV, Aguilar-Pimentel A, Klein-Rodewald T, Calzada-Wack J, Becker L, Marschall S, Kraiger M, Garrett L, Seisenberger C, Hölter SM, Borland K, Van De Logt E, Jansen PWTC, Baltissen MP, Valenta M, Vermeulen M, Wurst W, Gailus-Durner V, Fuchs H, de Angelis MH, Rando OJ, Kellner SM, Bultmann S, Schneider R.
Genes & Development 2020 May; 34(9-10):715.
Application：WB-Ce, Mouse, Embryonic stem cells.
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The rRNA m6A methyltransferase METTL5 is involved in pluripotency and developmental programs.
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