MRPL32 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MRPL32.
Immunogen
A synthetic peptide corresponding to internal of human MRPL32.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total MRPL32 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells (Lane 1), MCF-7 cells (Lane 2) and HepG2 cells (Lane 3 and lane 4), using MRPL32 polyclonal antibody (Cat # PAB17562).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using MRPL32 polyclonal antibody (Cat # PAB17562).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MRPL32
Entrez GeneID
64983Protein Accession#
Q9BYC8Gene Name
MRPL32
Gene Alias
HSPC283, L32mt, MRP-L32, bMRP-59b
Gene Description
mitochondrial ribosomal protein L32
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq
Other Designations
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