MRPL14 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells (Lane 1), COLO cells (Lane 2) and 293 cells (Lane 3 and lane 4), using MRPL14 polyclonal antibody (Cat # PAB17559).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MRPL14.
Immunogen
A synthetic peptide corresponding to internal of human MRPL14.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total MRPL14 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells (Lane 1), COLO cells (Lane 2) and 293 cells (Lane 3 and lane 4), using MRPL14 polyclonal antibody (Cat # PAB17559).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MRPL14
Entrez GeneID
64928Protein Accession#
Q6P1L8Gene Name
MRPL14
Gene Alias
L14mt, MGC70566, MRP-L14, MRP-L32, MRPL32, RMPL32, RPML32
Gene Description
mitochondrial ribosomal protein L14
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 17p13.3. [provided by RefSeq
Other Designations
39S ribosomal protein L14, mitochondrial|OTTHUMP00000016500
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