COL4A4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of COL4A4.
Immunogen
A synthetic peptide corresponding to internal of human COL4A4.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total COL4A4 protein.
Form
Liquid
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:100-1:500)
ELISA (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using COL4A4 polyclonal antibody (Cat # PAB17512).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of COS-7 cells, using COL4A4 polyclonal antibody (Cat # PAB17512).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — COL4A4
Entrez GeneID
1286Protein Accession#
P53420Gene Name
COL4A4
Gene Alias
CA44
Gene Description
collagen, type IV, alpha 4
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq
Other Designations
Collagen IV, alpha-4 polypeptide|OTTHUMP00000195046|alpha 4 type IV collagen|collagen of basement membrane, alpha-4 chain
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Interactome
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Disease
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