SMURF2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The SMURF2 polyclonal antibody (Cat # PAB1749) is used in Western blot to detect SMURF2 in mouse brain tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SMURF2 polyclonal antibody (Cat # PAB1749) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SMURF2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 702-731 of human SMURF2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA
Immunohistochemistry (1:50~100)
Immunofluorescence (1:10~50)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The SMURF2 polyclonal antibody (Cat # PAB1749) is used in Western blot to detect SMURF2 in mouse brain tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SMURF2 polyclonal antibody (Cat # PAB1749) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMURF2
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Interactome
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Pathway
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Publication Reference
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Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase.
Zhang Y, Chang C, Gehling DJ, Hemmati-Brivanlou A, Derynck R.
PNAS 2001 Jan; 98(3):974.
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Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation.
Kavsak P, Rasmussen RK, Causing CG, Bonni S, Zhu H, Thomsen GH, Wrana JL.
Molecular Cell 2000 Dec; 6(6):1365.
Application：IF, WB-Tr, Human, HEK 293T, Mv1Lu, U4A/Jak1 cells.
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Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling.
Lin X, Liang M, Feng XH.
The Journal of Biological Chemistry 2000 Nov; 275(47):36818.
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Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase.
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